<GBSet>
<GBSeq>
  <GBSeq_locus>AF485783</GBSeq_locus>
  <GBSeq_length>14758</GBSeq_length>
  <GBSeq_strandedness>double</GBSeq_strandedness>
  <GBSeq_moltype>DNA</GBSeq_moltype>
  <GBSeq_topology>circular</GBSeq_topology>
  <GBSeq_division>SYN</GBSeq_division>
  <GBSeq_update-date>15-MAY-2003</GBSeq_update-date>
  <GBSeq_create-date>21-MAR-2002</GBSeq_create-date>
  <GBSeq_definition>Binary vector pBI121, complete sequence</GBSeq_definition>
  <GBSeq_primary-accession>AF485783</GBSeq_primary-accession>
  <GBSeq_accession-version>AF485783.1</GBSeq_accession-version>
  <GBSeq_other-seqids>
    <GBSeqid>gb|AF485783.1|</GBSeqid>
    <GBSeqid>gi|19569229</GBSeqid>
  </GBSeq_other-seqids>
  <GBSeq_source>Binary vector pBI121</GBSeq_source>
  <GBSeq_organism>Binary vector pBI121</GBSeq_organism>
  <GBSeq_taxonomy>other sequences; artificial sequences; vectors</GBSeq_taxonomy>
  <GBSeq_references>
    <GBReference>
      <GBReference_reference>1</GBReference_reference>
      <GBReference_position>1..14758</GBReference_position>
      <GBReference_authors>
        <GBAuthor>Chen,P.Y.</GBAuthor>
        <GBAuthor>Wang,C.K.</GBAuthor>
        <GBAuthor>Soong,S.C.</GBAuthor>
        <GBAuthor>To,K.Y.</GBAuthor>
      </GBReference_authors>
      <GBReference_title>Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants</GBReference_title>
      <GBReference_journal>Mol. Breed. 11, 287-293 (2003)</GBReference_journal>
    </GBReference>
    <GBReference>
      <GBReference_reference>2</GBReference_reference>
      <GBReference_position>1..14758</GBReference_position>
      <GBReference_authors>
        <GBAuthor>To,K.Y.</GBAuthor>
      </GBReference_authors>
      <GBReference_title>Direct Submission</GBReference_title>
      <GBReference_journal>Submitted (20-FEB-2002) Institute of BioAgricultural Sciences, Academia Sinica, Taipei 11529, Taiwan</GBReference_journal>
    </GBReference>
  </GBSeq_references>
  <GBSeq_feature-table>
    <GBFeature>
      <GBFeature_key>source</GBFeature_key>
      <GBFeature_location>1..14758</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>1</GBInterval_from>
          <GBInterval_to>14758</GBInterval_to>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>organism</GBQualifier_name>
          <GBQualifier_value>Binary vector pBI121</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>mol_type</GBQualifier_name>
          <GBQualifier_value>genomic DNA</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>db_xref</GBQualifier_name>
          <GBQualifier_value>taxon:189807</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>constructed using pB221 from Clontech Laboratories and Bin19 described in GenBank Accession Number U09365</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(13..796)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>796</GBInterval_from>
          <GBInterval_to>13</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>similar to traF in GenBank Accession Number X54459</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>rep_origin</GBFeature_key>
      <GBFeature_location>complement(790..1168)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>1168</GBInterval_from>
          <GBInterval_to>790</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>ColE1 ori; similar to sequence in GenBank Accession Number V00268</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(1161..2344)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>2344</GBInterval_from>
          <GBInterval_to>1161</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>similar to tetA in GenBank Accession Number X75761</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(2454..2478)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>2478</GBInterval_from>
          <GBInterval_to>2454</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>T-DNA right border</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>promoter</GBFeature_key>
      <GBFeature_location>2519..2825</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>2519</GBInterval_from>
          <GBInterval_to>2825</GBInterval_to>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>NOS</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>gene</GBFeature_key>
      <GBFeature_location>2838..3632</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>2838</GBInterval_from>
          <GBInterval_to>3632</GBInterval_to>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>gene</GBQualifier_name>
          <GBQualifier_value>nptII</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>CDS</GBFeature_key>
      <GBFeature_location>2838..3632</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>2838</GBInterval_from>
          <GBInterval_to>3632</GBInterval_to>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>gene</GBQualifier_name>
          <GBQualifier_value>nptII</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>codon_start</GBQualifier_name>
          <GBQualifier_value>1</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>transl_table</GBQualifier_name>
          <GBQualifier_value>1</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>product</GBQualifier_name>
          <GBQualifier_value>neomycin phosphotransferase II</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>protein_id</GBQualifier_name>
          <GBQualifier_value>AAL92039.1</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>db_xref</GBQualifier_name>
          <GBQualifier_value>GI:19569230</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>translation</GBQualifier_name>
          <GBQualifier_value>MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>terminator</GBFeature_key>
      <GBFeature_location>4022..4277</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>4022</GBInterval_from>
          <GBInterval_to>4277</GBInterval_to>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>NOS</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>promoter</GBFeature_key>
      <GBFeature_location>4974..5808</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>4974</GBInterval_from>
          <GBInterval_to>5808</GBInterval_to>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>CaMV 35S</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>gene</GBFeature_key>
      <GBFeature_location>5845..7656</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>5845</GBInterval_from>
          <GBInterval_to>7656</GBInterval_to>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>gene</GBQualifier_name>
          <GBQualifier_value>gusA</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>CDS</GBFeature_key>
      <GBFeature_location>5845..7656</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>5845</GBInterval_from>
          <GBInterval_to>7656</GBInterval_to>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>gene</GBQualifier_name>
          <GBQualifier_value>gusA</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>GUS</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>codon_start</GBQualifier_name>
          <GBQualifier_value>1</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>transl_table</GBQualifier_name>
          <GBQualifier_value>1</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>product</GBQualifier_name>
          <GBQualifier_value>beta-glucuronidase</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>protein_id</GBQualifier_name>
          <GBQualifier_value>AAL92040.1</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>db_xref</GBQualifier_name>
          <GBQualifier_value>GI:19569231</GBQualifier_value>
        </GBQualifier>
        <GBQualifier>
          <GBQualifier_name>translation</GBQualifier_name>
          <GBQualifier_value>MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>terminator</GBFeature_key>
      <GBFeature_location>7727..7979</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>7727</GBInterval_from>
          <GBInterval_to>7979</GBInterval_to>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>NOS</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(8621..8646)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>8646</GBInterval_from>
          <GBInterval_to>8621</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>T-DNA left border</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(9156..10198)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>10198</GBInterval_from>
          <GBInterval_to>9156</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>similar to tetA in GenBank Accession Number L13842</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(10199..11680)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>11680</GBInterval_from>
          <GBInterval_to>10199</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>similar to trfA in GenBank Accession Number X00713</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(11681..12673)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>12673</GBInterval_from>
          <GBInterval_to>11681</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>similar to NPTIII gene in GenBank Accession Number V01547</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(12674..13443)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>13443</GBInterval_from>
          <GBInterval_to>12674</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>similar to transposable element IS1 in GenBank Accession Number X58999</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(13444..13794)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>13794</GBInterval_from>
          <GBInterval_to>13444</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>similarity to NPT III gene in GenBank Accession Number V01547</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>misc_feature</GBFeature_key>
      <GBFeature_location>complement(13795..14066)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>14066</GBInterval_from>
          <GBInterval_to>13795</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>similar to kilA in GenBank Accession Number M62846</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
    <GBFeature>
      <GBFeature_key>rep_origin</GBFeature_key>
      <GBFeature_location>complement(14141..14758)</GBFeature_location>
      <GBFeature_intervals>
        <GBInterval>
          <GBInterval_from>14758</GBInterval_from>
          <GBInterval_to>14141</GBInterval_to>
          <GBInterval_iscomp value="true"></GBInterval_iscomp>
          <GBInterval_accession>AF485783.1</GBInterval_accession>
        </GBInterval>
      </GBFeature_intervals>
      <GBFeature_quals>
        <GBQualifier>
          <GBQualifier_name>note</GBQualifier_name>
          <GBQualifier_value>ori V; similar to sequence in GenBank Accession Number M20134</GBQualifier_value>
        </GBQualifier>
      </GBFeature_quals>
    </GBFeature>
  </GBSeq_feature-table>
  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</GBSeq>

</GBSet>